Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.199681 |
Chromosome: | chromosome_12 |
Location: | 8335426 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g549550 | RSP19,TUB2 | Beta tubulin 2 | sense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CACCTAGATCACTACCACTTCTACACAGGC |
Internal bar code: | TCACAGTGCGTGAAGGGGGCGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 679 |
LEAP-Seq percent confirming: | 99.5968 |
LEAP-Seq n confirming: | 247 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TAACTTATGGAGCGCCTGCT |
Suggested primer 2: | CAAAAGGGAAGGGAAGAAGG |