| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.199756 |
| Chromosome: | chromosome 17 |
| Location: | 5294226 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g736250 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTGGAACCGCTCTTTTGGAGCTTCTGCA |
| Internal bar code: | GCCGCGGCGGGTTGCAGGCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 763 |
| LEAP-Seq percent confirming: | 99.0955 |
| LEAP-Seq n confirming: | 986 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCAACTCTGTCACGGCAAA |
| Suggested primer 2: | GAGGGAGGGGTATTCAGAGC |