Insertion junction: LMJ.RY0402.200815_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.200815
Chromosome:chromosome 12
Location:1195116
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g489700 OTC1 Ornithine carbamoyltransferase; (1 of 1) 2.1.3.3 - Ornithine carbamoyltransferase / OTCase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGAGATTTAACGGATCATGGGATTCTGTG
Internal bar code:CGGCTCTGCCGGAAGTTAATAT

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:0.0
LEAP-Seq n confirming:0
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:0

Suggested primers for confirmation by PCR

Suggested primer 1:TAGCGCTAGGCATGAAACCT
Suggested primer 2:GTGCACATGCTGACATACCC