Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.201100 |
Chromosome: | chromosome_7 |
Location: | 1245177 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre07.g321400 | FAP113 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AGCCCATTGTGTTCTCCACCCCATTGCGGT |
Internal bar code: | AACTAAGCATTCATGATGACCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 76 |
LEAP-Seq percent confirming: | 99.3579 |
LEAP-Seq n confirming: | 619 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTCGTAATCGTGCTCGTCAG |
Suggested primer 2: | GAAGGAAAGGAACGAGGGAC |