Insertion junction: LMJ.RY0402.201100_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.201100
Chromosome:chromosome 7
Location:1245177
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre07.g321400 MAW6,FAP113 (1 of 1) PF13948 - Domain of unknown function (DUF4215) (DUF4215); Membrane-associated hydroxyproline-rich glycoprotein 6 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCCCATTGTGTTCTCCACCCCATTGCGGT
Internal bar code:AACTAAGCATTCATGATGACCC

Confirmation - LEAP-Seq

LEAP-Seq distance:76
LEAP-Seq percent confirming:99.3579
LEAP-Seq n confirming:619
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TTCGTAATCGTGCTCGTCAG
Suggested primer 2:GAAGGAAAGGAACGAGGGAC