| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.201311 |
| Chromosome: | chromosome 12 |
| Location: | 7984731 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g552050 | (1 of 11) PF13229 - Right handed beta helix region (Beta_helix) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTCAGGCAGAGGACCGCTGATTGTAAGC |
| Internal bar code: | TACCACATCACATTAGATCATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 691 |
| LEAP-Seq percent confirming: | 99.7318 |
| LEAP-Seq n confirming: | 3718 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTCTTTTGTCGGCTACTG |
| Suggested primer 2: | CACACAGGTGGTCATCAAGG |