| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.201551 |
| Chromosome: | chromosome 12 |
| Location: | 4746685 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g523850 | MPPB,QCR1 | (1 of 1) K17732 - mitochondrial-processing peptidase subunit beta [EC:3.4.24.64] (PMPCB, MAS1); Ubiquinol:cytochrome c oxidoreductase 50 kDa core 1 subunit | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCCAGTTGCTGGTCTGCTCCGACCTGAG |
| Internal bar code: | GCCTCAGGTGGCGACGCTTACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 338 |
| LEAP-Seq percent confirming: | 99.52 |
| LEAP-Seq n confirming: | 622 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTTTGTTGCCTGGCTCTCT |
| Suggested primer 2: | ACCTGAACGTTCTCCACCAC |