Insertion junction: LMJ.RY0402.201797_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.201797
Chromosome:chromosome 11
Location:1319457
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g467712 SAGA1 StArch Granules Abnormal 1; (1 of 3) IPR000104//IPR002044//IPR013784 - Antifreeze protein, type I // Carbohydrate binding module family 20 // Carbohydrate-binding-like fold intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCAGTGCTGAAACAAACATATCCCCACCA
Internal bar code:GGGCGGACCAGAGGTGGATGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:420
LEAP-Seq percent confirming:95.0472
LEAP-Seq n confirming:403
LEAP-Seq n nonconfirming:21
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GTCGCTCAACTAGGGCTTTG
Suggested primer 2:GAAGTTCTCCTGGATCTGCG