Insertion junction: LMJ.RY0402.202173_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.202173
Chromosome:chromosome 2
Location:3707446
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g095200 (1 of 3) PTHR19211:SF15 - ATP-BINDING CASSETTE SUB-FAMILY F MEMBER 2 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTGCACCTGGCGCTTGGGTGATTGCTGTG
Internal bar code:AGCCTCCATGTTACGGTCGCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1172
LEAP-Seq percent confirming:98.618
LEAP-Seq n confirming:999
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:ACCAGGCGTACCTTCATGTC
Suggested primer 2:GCACGTGTGCTTGACTGTTT