Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.202444 |
Chromosome: | chromosome_12 |
Location: | 4046338 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g517400 | RAB5,RABF1 | Small Rab-related GTPase | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTCACTAACCCCTCCGTCAATCCAACATAG |
Internal bar code: | CCTTTCTACATTCAGGGTGTGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 257 |
LEAP-Seq percent confirming: | 99.6674 |
LEAP-Seq n confirming: | 1798 |
LEAP-Seq n nonconfirming: | 6 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTGTGGTGATGTGGTCCTTG |
Suggested primer 2: | GCAAGATGCAGTATGGCTGA |