| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.202811 |
| Chromosome: | chromosome 13 |
| Location: | 5195133 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g608000 | CPLD52 | Conserved in the Plant Lineage and Diatoms; (1 of 1) PTHR10366//PTHR10366:SF389 - NAD DEPENDENT EPIMERASE/DEHYDRATASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCAAACTGTAATACCATAATGTGGGCCGT |
| Internal bar code: | TGAAGTTTTTCCCGCTACACAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 996 |
| LEAP-Seq percent confirming: | 98.6425 |
| LEAP-Seq n confirming: | 218 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACTGGCAGGTTTTGGTTTG |
| Suggested primer 2: | GTTCGTTGCACCCTTACGAT |