Insertion junction: LMJ.RY0402.203532_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.203532
Chromosome:chromosome 11
Location:988832
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g467665 (1 of 1) IPR002909//IPR006626//IPR011050//IPR014756//IPR019316 - IPT domain // Parallel beta-helix repeat // Pectin lyase fold/virulence factor // Immunoglobulin E-set // G8 domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCTTCTCTGTTGGGCCCGACGGGGGATCC
Internal bar code:TCCAGTGCTCGCATGGTCCTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:328
LEAP-Seq percent confirming:98.9583
LEAP-Seq n confirming:285
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GAACCGTCCAGGTCTTTGAA
Suggested primer 2:ACTTCCTGCTCTCCCAGTCA