| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.203780 |
| Chromosome: | chromosome 16 |
| Location: | 2919195 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g663900 | HMBS,PBGD1 | (1 of 2) 2.5.1.61 - Hydroxymethylbilane synthase / Uroporphyrinogen synthetase; Porphobilinogen deaminase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGTGAATTTACACCAGACACAACAATCAC |
| Internal bar code: | ACCGTGCCATACTTCGTACTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1027 |
| LEAP-Seq percent confirming: | 99.5392 |
| LEAP-Seq n confirming: | 1080 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGAGTTTGTGACGCTCTGG |
| Suggested primer 2: | TCACCTCTTTCGTGCTCCTT |