| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.204074 |
| Chromosome: | chromosome 12 |
| Location: | 824674 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g495951 | CYN8,CYN20C,CYN20-3,ROC4 | (1 of 4) K03768 - peptidyl-prolyl cis-trans isomerase B (cyclophilin B) [EC:5.2.1.8] (PPIB, ppiB); Cyclophilin 20-3 | 3'UTR|outside_mRNA |
| Cre12.g495952 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGAAGTCATGCTTAGCACAGACGTCTGTG |
| Internal bar code: | GAGACAAAGAAGTCAGAAAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 884 |
| LEAP-Seq percent confirming: | 99.5907 |
| LEAP-Seq n confirming: | 730 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACCGGTTGCAAGTAGGTG |
| Suggested primer 2: | AACTGCCCCAAACTATGCAC |