| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.205015 |
| Chromosome: | chromosome 9 |
| Location: | 849341 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401700 | (1 of 1) PTHR16193:SF0 - TETRATRICOPEPTIDE REPEAT PROTEIN 27 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAGGAGGCGGGGACAAAAAAACATAAAAC |
| Internal bar code: | GATGGTACGGAATTTCAATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 551 |
| LEAP-Seq percent confirming: | 86.5331 |
| LEAP-Seq n confirming: | 7473 |
| LEAP-Seq n nonconfirming: | 1163 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTGTGCATGTGTGTGTGC |
| Suggested primer 2: | CCATACACGCGTACGATCAC |