| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.205116 |
| Chromosome: | chromosome 12 |
| Location: | 7097736 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g559350 | SDR23 | Short-chain dehydrogenase/reductase; (1 of 1) 1.1.1.101 - Acylglycerone-phosphate reductase / Palmitoyldihydroxyacetone-phosphate reductase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAAGCCTGTAACCCCACGGTGTGTGTGTG |
| Internal bar code: | CGTTTGAGTCCTGCCTGCCGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 714 |
| LEAP-Seq percent confirming: | 40.9077 |
| LEAP-Seq n confirming: | 4002 |
| LEAP-Seq n nonconfirming: | 5781 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCCTGCTCTCACCTGCCTA |
| Suggested primer 2: | GGAGCGATGTGTATGTGGTG |