Insertion junction: LMJ.RY0402.205326_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.205326
Chromosome:chromosome 4
Location:3788080
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g229550 SRR15 Scavenger receptor cysteine rich (SRCR) protein; (1 of 5) 1.4.3.13 - Protein-lysine 6-oxidase / Lysyl oxidase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTTTACCGTCCTGCCGGGCGAACGCCAAC
Internal bar code:AGGTTCATCTTTCGGTTACAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:234
LEAP-Seq percent confirming:98.7564
LEAP-Seq n confirming:11118
LEAP-Seq n nonconfirming:140
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CAGAGCTGCACCACCATCTA
Suggested primer 2:CTCAGCAGCAGTAGCAGCAC