| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.205371 |
| Chromosome: | chromosome 5 |
| Location: | 2500961 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g235700 | (1 of 2) K12382 - saposin (PSAP, SGP1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAAAGGCAGCTGGCTGACGTGGAACAAAC |
| Internal bar code: | CGGCAGAGAGGTAAGGCGAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 782 |
| LEAP-Seq percent confirming: | 99.6251 |
| LEAP-Seq n confirming: | 1860 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCACCTCGTCACTACATGC |
| Suggested primer 2: | TTCAGAGCGACACACGAAAC |