| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.205828 |
| Chromosome: | chromosome 12 |
| Location: | 3455191 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g496350 | SSD2 | Sterol sensing 5-transmembrane protein; (1 of 1) PF02460//PF12349 - Patched family (Patched) // Sterol-sensing domain of SREBP cleavage-activation (Sterol-sensing) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCGAGTGGACACGGCGGTGCGTGCCATC |
| Internal bar code: | GACCCATGTATTCTCCAAGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 236 |
| LEAP-Seq percent confirming: | 98.4076 |
| LEAP-Seq n confirming: | 309 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAGATGGACAGAAGCAAC |
| Suggested primer 2: | ATGGGTGAGTCTTTGAACCG |