| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.206089 |
| Chromosome: | chromosome 1 |
| Location: | 4531106 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g030850 | POA4 | 20S proteasome alpha subunit D; (1 of 1) K02731 - 20S proteasome subunit alpha 4 (PSMA7) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCCGTTAATGTGACCTTGAAACTCTGAA |
| Internal bar code: | TCCATACAATCGTGTGGGTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 665 |
| LEAP-Seq percent confirming: | 92.0302 |
| LEAP-Seq n confirming: | 3291 |
| LEAP-Seq n nonconfirming: | 285 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGTCCCTTCTTGCTACGC |
| Suggested primer 2: | GGGGGAGGGGTTGTAGATAA |