| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.206206 |
| Chromosome: | chromosome 7 |
| Location: | 2481706 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g329300 | MSC1 | Mechanosensitive ion channel; (1 of 1) PTHR30221 - SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAACCACCCTCCCCCACCACTTCACCCTT |
| Internal bar code: | CGCAAAGCCTTGACTCGGCTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 771 |
| LEAP-Seq percent confirming: | 93.9177 |
| LEAP-Seq n confirming: | 525 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATGAAGTTCTGCACCAGC |
| Suggested primer 2: | GCGTCTCCTATTGGAGCTTG |