| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.206304 |
| Chromosome: | chromosome 4 |
| Location: | 1261319 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g215050 | CHYB,CHYB1,CBH1 | Beta-carotene hydroxylase, putative chloroplast precursor | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGAGGGGGGCTGGAGAGGAGGTGATGGAG |
| Internal bar code: | TCATGGTGCATAGATAGGTGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 405 |
| LEAP-Seq percent confirming: | 99.4984 |
| LEAP-Seq n confirming: | 3769 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCCAGAAGTCAGGACAGA |
| Suggested primer 2: | CCTTCCCCTACTGCCTCTCT |