Insertion junction: LMJ.RY0402.206449_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.206449
Chromosome:chromosome 3
Location:1429983
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g151250 LANC1,LAN1 (1 of 1) PF05147 - Lanthionine synthetase C-like protein (LANC_like); LanC lantibiotic synthetase component C-like protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTACTCTAACCTTGAGGGGGAATAGTGCC
Internal bar code:AATCGGGACGATCGAACCGGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:359
LEAP-Seq percent confirming:76.9826
LEAP-Seq n confirming:398
LEAP-Seq n nonconfirming:119
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:CTACCCCACCAAGATGGCTA
Suggested primer 2:CATCACGGAACTAACATGCG