Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.206700 |
Chromosome: | chromosome 4 |
Location: | 2268983 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre04.g220200 | (1 of 1) PTHR16254:SF13 - K(+) EFFLUX ANTIPORTER 1, CHLOROPLASTIC-RELATED | 5'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCTTTGGAGCGGGACAGATTAACCTGAA |
Internal bar code: | TGAGTTATGTCGTAATTTTTCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 461 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 925 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AAGATATCCAAATTGGGGGC |
Suggested primer 2: | CATGAAACACTGCCCATGTC |