| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.206700 |
| Chromosome: | chromosome 4 |
| Location: | 2268993 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g220200 | (1 of 1) PTHR16254:SF13 - K(+) EFFLUX ANTIPORTER 1, CHLOROPLASTIC-RELATED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTACGCAAGCGATGCCAAGTCCAAAGAAC |
| Internal bar code: | TCTTGAAACATCTGGCATGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 895 |
| LEAP-Seq percent confirming: | 97.9957 |
| LEAP-Seq n confirming: | 1369 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGATATCCAAATTGGGGGC |
| Suggested primer 2: | CATGAAACACTGCCCATGTC |