| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.207338 |
| Chromosome: | chromosome 2 |
| Location: | 1629484 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g085257 | COP4,CHR2,CSOB | Channelrhodopsin 2; (1 of 1) IPR001425//IPR029730 - Archaeal/bacterial/fungal rhodopsin // Archaeal/bacterial/fungal rhodopsin-like | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGAGGTGAGTCAGCGCTGACGCGAGTTTT |
| Internal bar code: | AAACCGAAGTGGCCCGTTCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 832 |
| LEAP-Seq percent confirming: | 99.322 |
| LEAP-Seq n confirming: | 2051 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTAGGAAAGGGAGGGTGG |
| Suggested primer 2: | TCGTTCTTCTTGATGACCCC |