| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.207677 |
| Chromosome: | chromosome 16 |
| Location: | 4168300 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g668900 | A3,MTA3 | Hypothetical protein; (1 of 2) PTHR11266:SF41 - PXMP2/4 FAMILY PROTEIN 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAGGACTGGGAGCGTAATTTTGCATCGAG |
| Internal bar code: | GTCGACATATTGTAGCAGTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 889 |
| LEAP-Seq percent confirming: | 98.0769 |
| LEAP-Seq n confirming: | 153 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTTTAGCCGTTTGCGAGAT |
| Suggested primer 2: | TCATCCGTGCATGTTTGTTT |