| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.207872 |
| Chromosome: | chromosome 12 |
| Location: | 4803018 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g524350 | HUS1 | DNA damage checkpoint protein; (1 of 1) K10903 - HUS1 checkpoint protein (HUS1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACCATATCTCTAACCCACTTGCATGGCAG |
| Internal bar code: | CGATCGCTAGTTGCTCTTTTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 626 |
| LEAP-Seq percent confirming: | 99.7607 |
| LEAP-Seq n confirming: | 4585 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGATGCATCTGGGTTGTTC |
| Suggested primer 2: | ATGAATCTAGCGCAGCAGGT |