| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.207934 |
| Chromosome: | chromosome 4 |
| Location: | 1587994 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g213300 | (1 of 88) IPR011333 - POZ domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCAATCAATCAATCGAAAGCTCTCTGACG |
| Internal bar code: | CAGCTCCAGGTGCCGAAACGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 734 |
| LEAP-Seq percent confirming: | 91.254 |
| LEAP-Seq n confirming: | 1732 |
| LEAP-Seq n nonconfirming: | 166 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCAAGGACTCACTGGGCT |
| Suggested primer 2: | GAGCCCAGAAACCAACACAT |