| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.208103 |
| Chromosome: | chromosome 7 |
| Location: | 5459619 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g350700 | RAA6 | psaA mRNA trans-splicing factor | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGAGGGGCGGCGGCGGCGGCGCGGGCGC |
| Internal bar code: | CGGAGGATGCGCAGGATGCGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 836 |
| LEAP-Seq percent confirming: | 99.506 |
| LEAP-Seq n confirming: | 1813 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACCGACGAACACATCACAC |
| Suggested primer 2: | GATGTGGATCCGACAGACCT |