| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.208464 |
| Chromosome: | chromosome 3 |
| Location: | 3599711 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g168450 | CCT8 | (1 of 1) K09500 - T-complex protein 1 subunit theta (CCT8); T-complex protein 1, theta subunit | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGTGTTGTGCATGCGCCTGAAAGAATTC |
| Internal bar code: | CACGGACCCGCGAGCGGTAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 485 |
| LEAP-Seq percent confirming: | 99.1736 |
| LEAP-Seq n confirming: | 1560 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGCTGCTGTTGTTGTTGT |
| Suggested primer 2: | ACAGCATCCTGGACCTGTTC |