| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.208464 |
| Chromosome: | chromosome 5 |
| Location: | 2177135 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234647 | (1 of 1) K06950 - uncharacterized protein (K06950) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACGAAGGAAGATACGAGCGCTGAGGGCGG |
| Internal bar code: | GGCGCGGCTTCTGTCGGGCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1037 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 1384 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGGCTTCGTTTGGTTGAC |
| Suggested primer 2: | ACCGGTGAGGGCTTTTAACT |