| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.208464 |
| Chromosome: | chromosome 7 |
| Location: | 2723160 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g331150 | (1 of 2) 1.3.3.5 - Bilirubin oxidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCATGCCCGAGCCCGTCGAGCCCGAGGAC |
| Internal bar code: | ACGCCCGTTGAGCACGCACTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 471 |
| LEAP-Seq percent confirming: | 99.7333 |
| LEAP-Seq n confirming: | 374 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGACGAAACCATACATGC |
| Suggested primer 2: | ATGTCCGACTACTGCAACCC |