Insertion junction: LMJ.RY0402.208586_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.208586
Chromosome:chromosome 16
Location:6153860
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g675550 FKB16-2,FKB5,FKB16B (1 of 2) PTHR10516//PTHR10516:SF281 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE // 12 KDA FK506-BINDING PROTEIN; Peptidyl-prolyl cis-trans isomerase, FKBP-type intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCTCATCCCCAGGTCACGCCTCTCAGCCA
Internal bar code:ACATTTGCTCAGAGGCGGAGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:309
LEAP-Seq percent confirming:99.7394
LEAP-Seq n confirming:1531
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGACTGAGCAAAACCCTC
Suggested primer 2:CCCCAACCAATGCTATAACG