| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.208715 |
| Chromosome: | chromosome 2 |
| Location: | 7423654 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g144550 | (1 of 4) PTHR10110:SF119 - SODIUM/HYDROGEN EXCHANGER 5-RELATED | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCGGGCCAGTGGCAGAGCACAGAGCGGT |
| Internal bar code: | GCAGCTTCTGCTCCGAAGCGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 908 |
| LEAP-Seq percent confirming: | 99.846 |
| LEAP-Seq n confirming: | 2594 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGCGGGGGATGATAATGT |
| Suggested primer 2: | ACGCAGCTAAGCATCCTCAT |