| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.209161 |
| Chromosome: | chromosome_13 |
| Location: | 4176176 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g592150 | SPL28,PRP28 | DEAD/DEAH box helicase, possible nuclear pre-mRNA splicing facto | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCCAGCCGCTGCCGCTGATTCATCACATTT |
| Internal bar code: | TATTCGGTCACATTGATCTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 284 |
| LEAP-Seq percent confirming: | 99.7382 |
| LEAP-Seq n confirming: | 4191 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGCACCAAACAGGTTATCT |
| Suggested primer 2: | CCATTCACCACTGATGTTGC |