Insertion junction: LMJ.RY0402.209178_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.209178
Chromosome:chromosome 11
Location:3575580
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g481750 GAS30 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Hydroxyproline-rich glycoprotein, stress-induced 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGAGTCTCTGTACCTGGACACTGCGGGTA
Internal bar code:CATGGGTGGCGACGACTGATCT

Confirmation - LEAP-Seq

LEAP-Seq distance:156
LEAP-Seq percent confirming:99.343
LEAP-Seq n confirming:756
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CTACCGGTACCGTCAAGCAT
Suggested primer 2:GAACTCAAGCTTTCGGGTTG