| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.209517 |
| Chromosome: | chromosome 13 |
| Location: | 334227 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g564350 | ATM1 | (1 of 1) K04728 - ataxia telangiectasia mutated family protein (ATM, TEL1); Ataxia telangiectasia mutated protein 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCCGCCGCCGTGGCCGCTCCCGACCGGC |
| Internal bar code: | AGCGTCCCGAAGAGACGAGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 390 |
| LEAP-Seq percent confirming: | 85.0 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGGCCTGGACATTGACTAC |
| Suggested primer 2: | TGAACCAATCCCGTAAGGAG |