| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.209685 |
| Chromosome: | chromosome 17 |
| Location: | 4067147 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g729250 | UPF2 | (1 of 1) K14327 - regulator of nonsense transcripts 2 (UPF2, RENT2); Putative UPF2 regulator of nonsense transcripts | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGTGCGTCGCCCGCCACACCACCCGTGG |
| Internal bar code: | GACGCTGGGACGCACCACCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 925 |
| LEAP-Seq percent confirming: | 46.1909 |
| LEAP-Seq n confirming: | 4123 |
| LEAP-Seq n nonconfirming: | 4803 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAACCAGCCAAGCCAGAAC |
| Suggested primer 2: | TGCGCTTGTATCTCCTTGTG |