| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.209795 |
| Chromosome: | chromosome 4 |
| Location: | 2957536 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g224800 | VAMP4,VAMP74,VAM4 | (1 of 3) PTHR21136:SF4 - N-SYNAPTOBREVIN, ISOFORM J; Endosomal R-SNARE protein, Vamp7/Nyv1-family (R.III) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATACGGCTGCTGTCTTCAATCGTCGGGTGC |
| Internal bar code: | GAGCCCATGGTAAGGCGCGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 771 |
| LEAP-Seq percent confirming: | 99.5291 |
| LEAP-Seq n confirming: | 6764 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTTTGGTTGCATGTTTGC |
| Suggested primer 2: | ACAAGGTTTTGTCCGTCAGG |