Insertion junction: LMJ.RY0402.209855_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.209855
Chromosome:chromosome 14
Location:1027302
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g614400 SRH23 (1 of 1) K15711 - SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A3 [EC:3.6.4.- 6.3.2.19] (SMARCA3, HLTF); SNF2-related DNA/RNA helicase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAATGTGAGTGTCAGGGATGGAGCACAAGG
Internal bar code:GGTTGATCGCATGGTTTGCAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:323
LEAP-Seq percent confirming:92.718
LEAP-Seq n confirming:1095
LEAP-Seq n nonconfirming:86
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:GACTTTTCAGCATCATCGCA
Suggested primer 2:GTTTTCTGCGTCTTCCTTGC