| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.210228 |
| Chromosome: | chromosome 12 |
| Location: | 8962231 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g544114 | CYN20-6 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCATAGTTTTGCACGGCAGAGAGCCAAGT |
| Internal bar code: | GACACACATAAGGAGGCGCTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 914 |
| LEAP-Seq percent confirming: | 99.5653 |
| LEAP-Seq n confirming: | 6184 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGTAACCGCCAGTACCAT |
| Suggested primer 2: | CGAGAAGAAGAATGGCCTTG |