Insertion junction: LMJ.RY0402.210740_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.210740
Chromosome:chromosome 11
Location:2161854
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre11.g469600 HDA9 (1 of 1) IPR000286//IPR002110//IPR020683//IPR023801 - Histone deacetylase superfamily // Ankyrin repeat // Ankyrin repeat-containing domain // Histone deacetylase domain; RPD3/HDA1 type histone deacetylase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATTCGCTGAGGTGGCGGCGCTGCAGCGGC
Internal bar code:TGTTACATGGCTATTTTCGTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:49
LEAP-Seq percent confirming:30.0
LEAP-Seq n confirming:24
LEAP-Seq n nonconfirming:56
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CACGGCTGCTGATACGAATA
Suggested primer 2:GGAGAACAAAGCGCAAAGAC