Insertion junction: LMJ.RY0402.210794_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.210794
Chromosome:chromosome 14
Location:2272759
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre14.g623200 LAP1 Leucyl aminopeptidase; (1 of 1) 3.4.11.1//3.4.11.23 - Leucyl aminopeptidase / Peptidase S // PepB aminopeptidase / Peptidase B intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCAACCCCACCACAACCCTTTCCATCCCA
Internal bar code:TCGGATCACCGACATCCTCTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:716
LEAP-Seq percent confirming:99.0188
LEAP-Seq n confirming:2422
LEAP-Seq n nonconfirming:24
LEAP-Seq n unique pos:37

Suggested primers for confirmation by PCR

Suggested primer 1:GGGTGTGGGGTCATGATTAG
Suggested primer 2:TAAACTAGCGGAGGGGTGTG