Insertion junction: LMJ.RY0402.210834_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.210834
Chromosome:chromosome 4
Location:675213
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g217951 LCI33 Low-CO2-inducible protein; (1 of 9) IPR011011//IPR013083 - Zinc finger, FYVE/PHD-type // Zinc finger, RING/FYVE/PHD-type intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAACACAAAATACATGCTAAACTCCCTTCT
Internal bar code:TTTTTGGAATCTTCCAGTAGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:151
LEAP-Seq percent confirming:99.5146
LEAP-Seq n confirming:205
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GAGTCAAAGCTAACCGCGTC
Suggested primer 2:TTTGCAACAAGCTCATCGAC