| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.210986 |
| Chromosome: | chromosome 14 |
| Location: | 1087156 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g614900 | TSQ1 | Glutaminyl-tRNA synthetase; (1 of 1) 6.1.1.18 - Glutamine--tRNA ligase / Glutaminyl-tRNA synthetase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAGATAAACTCCCACTCCATCCCACACC |
| Internal bar code: | ATTATTTATTGTGTTTAAGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 173 |
| LEAP-Seq percent confirming: | 99.6796 |
| LEAP-Seq n confirming: | 4045 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACCTGCAAGGACCCTACAA |
| Suggested primer 2: | CGTACCGCACGTTCACATAC |