| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.211051 |
| Chromosome: | chromosome_12 |
| Location: | 1361080 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g488450 | VSP3 | Extracellular matrix protein | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGATTACGTAGGCATCAGCGCCCGCAAGCT |
| Internal bar code: | GCACTACCGAATGGCTCTGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1009 |
| LEAP-Seq percent confirming: | 98.6183 |
| LEAP-Seq n confirming: | 1142 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTGTCAGACATCAGCAGT |
| Suggested primer 2: | CACCGCTCATGTCTTCGTAA |