Insertion junction: LMJ.RY0402.211306_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.211306
Chromosome:chromosome 11
Location:2164965
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g469600 HDA9 (1 of 1) IPR000286//IPR002110//IPR020683//IPR023801 - Histone deacetylase superfamily // Ankyrin repeat // Ankyrin repeat-containing domain // Histone deacetylase domain; RPD3/HDA1 type histone deacetylase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCGTCGTCTTCATCGTCTTCATCGTCTTC
Internal bar code:GAGACCGGATAGATGCCCGCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:146
LEAP-Seq percent confirming:98.75
LEAP-Seq n confirming:158
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GGGTGTCTGTGGGACTGTCT
Suggested primer 2:GAGAAGGAGGAGGATGGGTC