| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.211402 |
| Chromosome: | chromosome 12 |
| Location: | 9463707 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g539650 | (1 of 1) PF00530//PF08016 - Scavenger receptor cysteine-rich domain (SRCR) // Polycystin cation channel (PKD_channel) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCCTGCTTGTATCCCGTTCCCAATGTAA |
| Internal bar code: | AACAGCGGCTACCCCCCCCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 40 |
| LEAP-Seq percent confirming: | 88.0515 |
| LEAP-Seq n confirming: | 479 |
| LEAP-Seq n nonconfirming: | 65 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGAAGTGAAGGCGTAGGCA |
| Suggested primer 2: | AAGGTTTACCGCACATCTCG |