| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.211519 |
| Chromosome: | chromosome 10 |
| Location: | 2216481 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g433950 | (1 of 2) PTHR33222:SF3 - PROTEIN CURVATURE THYLAKOID 1C, CHLOROPLASTIC | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACATTGCATTGGAAAGGGGAGGCGGCGGC |
| Internal bar code: | CGTCAAACGTGCTCTAGAAAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 787 |
| LEAP-Seq percent confirming: | 99.3825 |
| LEAP-Seq n confirming: | 2575 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAAAGTCCGAGAAAGACG |
| Suggested primer 2: | GGACATTATCGCGTGGTTCT |