Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.212377 |
Chromosome: | chromosome_14 |
Location: | 475476 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre14.g611100 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTGCCGTGAGACCGGCCTCGTCCTCACCCC |
Internal bar code: | GAAGATTTTAGATTTTTGGGCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 223 |
LEAP-Seq percent confirming: | 99.0596 |
LEAP-Seq n confirming: | 316 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACGTAGGTCCTCCCCCATAC |
Suggested primer 2: | GCTGGCAATGTGGTTATGTG |